Debenhams administrators have stated that 320 employees at the company will be affected as it has decided to permanently close stores in six cities.
The store on Oxford Street in London will be closed along with offices in Portsmouth, Staines, Harrogate, Weymouth in Dorset and Worcester.
The company launched a liquidation process last month after failing to complete a last-minute rescue sale.
Debenhams’ administrator, FRP Advisory, said it would continue to speak to third parties about the potential sales of all or part of the historic retail store.
Geoff Rowley, Debenhams Joint Administrator and Partner at FRP Advisory, said: “We continue to work with interested parties on alternative proposals for the future of Debenhams.
“The recent lockdown has inevitably affected our plans to wind up the business.
“We regret the impact on colleagues affected by today’s announcement and we would like to thank everyone who continues trading under very difficult circumstances.”