Boris Johnson suffered a sizeable background uprising when 35 Conservative MPs opposed extending the coronavirus laws for another six months.
The prime minister had ordered his MPs to support an extension to wide-ranging powers in the coronavirus law.
However, some ignored it and voted against it, including former Cabinet Ministers David Davis and Esther McVey.
Conservative MP Chris Green (Bolton West) acted as narrator for the Noes, also a sign of rebellion.
For Labor, 21 Labor MPs rebelled to oppose the extension – including former Shadow Chancellor John McDonnell and former hopeful leader Rebecca Long-Bailey.
Health Secretary Matt Hancock stated that some of the powers of the law need to be renewed every six months, including measures to protect tenants from eviction and to provide statutory sick pay to people during periods of self-isolation.
Parts of the legislation were allowed to expire, but concerns were raised about powers that could affect the right to protest.
The extension of the measures in the law was approved by 484 to 76, majority 408.
The division list showed that there were 305 Conservative MPs and 176 Labor MPs among those who supported them.
A total of 10 Liberal Democrats voted against the extension, with former minister Alistair Carmichael also serving as cashier.
Seven DUP MPs, Alliance MP Stephen Farry (North Down), the Green MP, Caroline Lucas (Brighton Pavilion) and former union leader Jeremy Corbyn, who is currently an Independent, also opposed the measures.
The 35 conservative rebels were:
They were: Adam Afriyie (Windsor), Steve Baker (Wycombe), Harriett Baldwin (West Worcestershire), Bob Blackman (Harrow East), Peter Bone (Wellingborough), Graham Brady (Altrincham and Sale West), Christopher Chope (Christchurch), Geoffrey Clifton-Brown (The Cotswolds), Philip Davies (Shipley), David Davis (Haltemprice and Howden), Jonathan Djanogly (Huntingdon), Richard Drax (South Dorset), Mark Francois (Rayleigh and Wickford), Marcus Fysh (Yeovil), Mark Harper (Forest of Dean), Philip Hollobone (Kettering), David Jones (Clwyd West), Pauline Latham (Mid Derbyshire), Jonathan Lord (Woking), Tim Loughton (East Worthing and Shoreham), Craig Mackinlay (South Thanet), Karl McCartney (Lincoln), Stephen McPartland (Stevenage), Esther McVey (Tatton), Anne Marie Morris (Newton Abbot), John Redwood (Wokingham), Andrew Rosindell (Romford), Henry Smith (Crawley), Julian Sturdy (York Outer) , Desmond Swayne (New Forest West), Robert Syms (Poole), Craig Tracey (North Warwickshire), Charles Walker ( B. Roxbourne), David Warburton (Somerton and Frome), William Wragg (Hazel Grove).